| 牟微娜,李 苗,谢长生.别直参抑制人乳腺癌细胞MCF-7体外增殖[J].中国肿瘤,2016,25(12):1004-1008. |
| 别直参抑制人乳腺癌细胞MCF-7体外增殖 |
| Anti-proliferative Effect of Korean Ginseng on Human Breast Cancer Cell MCF-7 |
| 投稿时间：2016-02-22 |
| DOI：10.11735/j.issn.1004-0242.2016.12.A012 |
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| 中文关键词: 人参 MCF-7细胞 细胞增殖 bax/bcl-2 |
| 英文关键词:Ginseng MCF-7 cells cell proliferation bax/bcl-2 |
| 基金项目:浙江省中医药科技计划项目（2012ZB041） |
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| 中文摘要: |
| 摘 要：［目的］ 评价别直参对MCF-7细胞体外增殖的影响，并检测其对凋亡相关蛋白bax/bcl-2表达的影响。［方法］ 采用MTT比色法检测对MCF-7细胞增殖的作用；流式细胞术检测对MCF-7细胞凋亡的影响；免疫组化法检测bax/bcl-2的表达。［结果］ 别直参高剂量组作用MCF-7细胞24h后，MTT检测增殖率为89.83%，提示有抑制MCF-7细胞体外增殖的作用（P<0.05）；流式细胞术检测高剂量组细胞凋亡率明显升高（P<0.05）；免疫组化法检测高剂量组能上调MCF-7细胞bax的表达，并下调bcl-2的表达。［结论］ 别直参高剂量组具有一定的诱导MCF-7细胞凋亡效应作用，其机制可能通过上调bax的表达和下调bcl-2的表达。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the impact of serum containing Korean Ginseng on MCF-7 cell proliferation and the expression of bax/bal-2 proteins. ［Methods］ By using the MTT method to detect cell proliferation and flow cytometry to examine cell apoptosis，while the bax/bcl-2 proteins were detected by IHC. ［Results］ The high dose of Korean ginseng could make an anti-proliferative effect on MCF-7 cell with the proliferation rate of 89.83% by MTT method as well as high apoptosis rate by flow cytometry（P<0.05）. The IHC revealed high dose of Korean ginseng increase the expression of bax protein and decrease the expression of bcl-2 protein. ［Conclusion］ The high dose of Korean ginseng induced apoptosis in MCF-7 cell by increasing the expression of bax protein and decreasing the expression of bcl-2 protein. |
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