| 罗福永,刘 颖,朱星华.小剂量阿糖胞苷诱导神经母细胞瘤细胞分化的研究[J].中国肿瘤,2016,25(11):904-909. |
| 小剂量阿糖胞苷诱导神经母细胞瘤细胞分化的研究 |
| Study of Differentiation of Neuroblastoma SMS-KCNR cell by Low-dose Cytosine Arabinside |
| 投稿时间：2016-06-18 |
| DOI：10.11735/j.issn.1004-0242.2016.11.A014 |
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| 中文关键词: 神经母细胞瘤 SMS-KCNR细胞 小剂量阿糖胞苷 诱导分化 |
| 英文关键词:neuroblastoma SMS-KCNR cell low-dose Cytosine arabinsidel induce differentiation |
| 基金项目:沈阳市科学技术计划项目（F13-221-9-43） |
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| 中文摘要: |
| 摘 要：［目的］ 通过人神经母细胞瘤(NB)SMS-KCNR细胞的体外实验，观察小剂量阿糖胞苷(Ara-C)对NB细胞的诱导分化作用。［方法］ MTS法检测小剂量Ara-C对SMS-KCNR细胞增殖的影响；观察小剂量Ara-C处理后SMS-KCNR细胞的形态变化；流式细胞仪检测小剂量Ara-C处理后SMS-KCNR细胞的细胞周期分布；Western blot检测TrkA、N-myc蛋白的表达，RT-PCR检测TrkA及MYCN mRNA水平的变化。［结果］ 小剂量Ara-C对SMS-KCNR细胞的增殖具有抑制作用，诱导SMS-KCNR细胞周期停滞于S期，G2/M期细胞比例下降 （P<0.05）。经小剂量Ara-C处理后SMS-KCNR细胞形态与正常神经节细胞类似，并使TrkA的表达上调（P<0.05），MYCN的表达下调（P<0.05）。［结论］ 小剂量Ara-C对SMS-KCNR细胞具有诱导分化作用，该作用可能与TrkA表达上调，MYCN表达下调有关。 |
| 英文摘要: |
| Abstract：［Purpose］ Through a series of experiments of SMS-KCNR cells of neuroblastoma (NB) by low-dose cytosine arabinside (Ara-C) in vitro. To explore the differentiation of SMS-KCNR cells by low-dose Ara-C. ［Methods］ The proliferation of SMS-KCNR cells treated with low-dose Ara-C was detected by MTS. The morphological changes of SMS-KCNR cells was observed by microscope. The distribution of cell cycles was analyzed by Flow cytometry. Further，the protein expression of TrkA and N-myc was measured by Western blot and the mRNA change of the TrkA and MYCN was quantified by the real time-PCR. ［Results］ The proliferation of SMS-KCNR cells were inhibited by low-dose Ara-C. Low-dose Ara-C induced the stagnation of the cell cycle in S phase. The content of SMS-KCNR cells in G2/M phase decreased (P<0.05). SMS-KCNR cells’ morphology was similar to normal ganglion cells after treated by low-dose Ara-C. The expression of TrkA increased by low-dose Ara-C. but decreased the expression of MYCN. ［Conclusion］ The low-dose Ara-C could induce the differentiation of SMS-KCNR cells might be through increased the expression of TrkA. |
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