| 吐尔更·吾那,郭 雨,张志强.c-Met siRNA 对食管鳞癌细胞CE81T-4生物学特性的影响[J].中国肿瘤,2016,25(9):721-727. |
| c-Met siRNA 对食管鳞癌细胞CE81T-4生物学特性的影响 |
| The Effect of siRNA c-Met on the Biological Characteristics of Esophageal Squamous Cell Carcinoma Cell CE81T-4 |
| 投稿时间：2016-01-15 |
| DOI：10.11735/j.issn.1004-0242.2016.09.A012 |
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| 中文关键词: 食管鳞癌 CE81T-4细胞 c-Met siRNA 增殖 迁移 侵袭 |
| 英文关键词:esophageal squamous cell carcinoma CE81T-4 cell c-Met siRNA proliferation migration invasion |
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| 中文摘要: |
| 摘 要：［目的］ 探讨c-Met siRNA 对食管鳞癌细胞CE81T-4生物学特性的影响。［方法］ 利用 siRNA技术，采用带有荧光的慢病毒包装的c-Met siRNA稳定转染CE81T-4，并设立转染慢病毒包装的c-Met siRNA的CE81T-4为实验组，转染空慢病毒作为阴性对照，未转染CE81T-4细胞作为空白对照。于荧光倒置显微镜下观察转染效果。采用qRT-PCR检测三组细胞中c-Met mRNA的表达量，Western blot检测c-Met蛋白表达。利用MTT、划痕实验、Transwell及流式细胞术分别检测细胞的增殖、迁移、侵袭、周期及凋亡。［结果］ 转染c-Met siRNA后，荧光倒置显微镜下可见广泛绿色荧光表达；实验组c-Met mRNA的表达量（0.1758±0.0150）显著低于阴性对照组（0.3384±0.0346）和空白对照组（0.3759±0.0252）（P<0.05）。实验组c-Met蛋白表达显影较空白对照组及阴性对照组低；实验组细胞的增殖能力显著降低，与阴性对照组和空白对照组比较差异有统计学意义（P<0.05）。Transwell体外侵袭实验提示实验组的侵袭迁移个数为135±11.79，显著低于空白对照组（186±14.98）及阴性对照组（178±12.53）（P<0.05）。实验组细胞凋亡率（17.87%±1.60%）高于阴性对照组（4.93%±2.49%）及空白对照组（4.37%±1.60%）（P<0.05）。实验组G0/G1期细胞比例（48.57%±4.91%）较阴性对照组（38.13%±3.23%）和空白对照组（37.07%±2.32%）显著增多（P<0.05）。［结论］ c-Met siRNA可增加食管鳞癌细胞的凋亡率，阻滞细胞周期在G0/G1期，降低细胞增殖、迁移及侵袭能力。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effects of siRNA c-Met on the biological characteristics of esophageal squamous cell carcinoma cell line CE81T-4. ［Methods］ The transfected CE81T-4 which c-Met siRNA was packaged by slow virus of fluorescence was stabled by siRNA technique. C-Met siRNA for transfection of CE81T-4 was established as the experimental group，transfection of empty slow virus as negative control，and non transfected esophageal squamous cell carcinoma CE81T-4 as normal control. The transfection effect was observed under the inverted fluorescence microscope. The expression of mRNA c-Met in three groups of cells was detected by qRT-PCR. C-Met protein expression was detected by Western blot. The proliferation，migration，invasion，cycle and apoptosis of cells were detected by MTT，scratch test，Transwell and flow cytometry respectively.［Results］ After transfection with siRNA c-Met，the expression of green fluorescence was observed under fluorescence inverted microscope. The expression level of c-Met mRNA in the experimental group（0.1758±0.0150） was significantly lower than that in the negative control group（0.3384±0.0346） and normal control group（0.3759±0.0252）（P<0.05）. The expression of c-Met protein in the experimental group was lower than that in the control group. The cell proliferation ability of the experimental group was significantly lower than that of the control groups（P<0.05）. Transwell in vitro invasion experiment showed that the number of invasion and migration of the experimental group（135±11.79） was significantly lower than that of the normal control group （186±14.98）and the negative control group（178±12.53）（P<0.05）. The apoptosis rate of the experimental group（17.87%±1.60%）was higher than that of the negative control group(4.93%±2.49%) and normal control group（4.37%±1.60%），with significant difference（P<0.05）. Compared with the negative control group (38.13%±3.23%) and normal control group (37.07%±2.32%)，the G0/G1 phase cells of the experimental group(48.57%±4.91%) was significantly increased(P<0.05).［Conclusion] siRNA c-Met can increase the apoptosis rate of esophageal squamous cell carcinoma cells，block the cycle in the G0/G1 phase，and reduce the proliferation，migration and invasion ability. |
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