| 史亚飞,刘启龙.水飞蓟素联合阿霉素对耐药细胞株MCF-7/ADM的逆转耐药作用[J].中国肿瘤,2016,25(8):649-652. |
| 水飞蓟素联合阿霉素对耐药细胞株MCF-7/ADM的逆转耐药作用 |
| Reversal Effect of Silymarin Combined with Adriamycin on Multidrug Resistant Cell Line MCF-7/ADM |
| 投稿时间：2015-12-04 |
| DOI：10.11735/j.issn.1004-0242.2016.08.A013 |
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| 中文关键词: 水飞蓟素 阿霉素 人乳腺癌细胞 耐药性 |
| 英文关键词:silymarin adriamycin breast cancer cell drug resistance |
| 基金项目:山东省教育厅课题(J13LK13)；济宁市科技局课题（2012jnwk02；2013jnwk77) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨水飞蓟素联合阿霉素对人乳腺癌耐药细胞MCF-7/ADM耐药性的影响。［方法］ 采用MTT法测定单用阿霉素及其联合水飞蓟素对MCF-7/S、MCF-7/ADM细胞增殖抑制率（IR），计算耐药倍数和逆转倍数。［结果］ 阿霉素作用MCF-7/S的IC50值（2.89±0.35μg/ml）明显小于MCF-7/ADM（56.85±2.12μg/ml），耐药倍数为27.6倍；阿霉素联合水飞蓟素作用MCF-7/ADM的IC50值（9.07±0.56μg/ml）明显小于单独使用阿霉素（59.52±2.38μg/ml），逆转耐药倍数为6.6倍。［结论］ 人乳腺癌MCF-7/ADM细胞对阿霉素具有明确耐药性，水飞蓟素能够明显逆转MCF-7/ADM对阿霉素的耐药性。 |
| 英文摘要: |
| Abstract：［Purpose］ To explore the effect of silymarin combined adriamycin on drug resistance of human breast cancer cells. ［Methods］ The inhibitory rates(IR) of MCF-7/ADM cells caused by single silymarin and combined adriamycin were detected by MMT assay，and the resistant index and reversal index were calculated．［Results］ IC50 of adriamycin to MCF-7/S （2.89±0.35μg/ml）was significant lower than that to MCF-7/ADM(56.85±2.12μg/ml). Drug-Resistant index of MCF-7/ADM was 27.6；IC50 of silymarin combined adriamycin to MCF-7/ADM （9.07±0.56μg/ml）was significant lower than that of adriamycin(59.52±2.38μg/ml)，and reversal index was 6.6. ［Conclusion］ Human breast cancer MCF-7/ADM cells have clear drug resistance to adriamycin. Silymarin reverses the resistance of MCF-7/ADM to adriamycin. |
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