| 王耀焓,张培彤,杨 栋.不同剂量川芎对PG干细胞样细胞低氧微环境改善的研究[J].中国肿瘤,2016,25(7):547-552. |
| 不同剂量川芎对PG干细胞样细胞低氧微环境改善的研究 |
| Rhizoma Chuanxiong Improves Hypoxia Microenvironment of PG-BE1 Tumor Stem-like Cells |
| 投稿时间：2015-11-05 |
| DOI：10.11735/j.issn.1004-0242.2016.07.A010 |
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| 中文关键词: 川芎 肿瘤干细胞 乏氧 |
| 英文关键词:Rhizome Chuanxiong cancer stem cells hypoxia microenvironment |
| 基金项目:国家自然科学基金（81173450；81302963） |
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| 摘要点击次数: 2060 |
| 全文下载次数: 1134 |
| 中文摘要: |
| 摘 要：［目的］ 观察体内实验中不同剂量川芎对肿瘤干细胞样细胞低氧微环境的影响。［方法］ 通过无血清培养获得的干细胞样细胞并鉴定，将已鉴定的球细胞接种于裸鼠腋下，将裸鼠平均随机分为3组，即对照组、高剂量川芎组、低剂量川芎组，21d后检测抑瘤率，Western blot检测瘤体ABCG2、HIF-1α蛋白表达，RT-PCR检测HIF-1α mRNA表达。［结果］低剂量川芎对PG-BE1干细胞样细胞具有杀伤作用，可在蛋白水平上抑制ABCG2的表达(P＜0.05)。低剂量川芎同时还可在蛋白及mRNA的水平上抑制HIF-1α的表达。［结论］低剂量川芎对肿瘤干细胞的杀伤作用可能与其抑制HIF-1α蛋白表达，改善肿瘤乏氧微环境有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To observe the effect of different doses of Rhizoma Chuanxiong on the hypoxia microenvironment of cancer stem-like cells. ［Methods］ Sphere PG-BE1 tumor stem-like cells were inoculated in nude mouse and the animals were randomly divided into 3 groups：control group，high-dose Rhizoma Chuanxiong group and low dose of Rhizoma Chuanxiong group. The tumor inhibition rate was detected after 21 days. Expression of ABCG2 and HIF-1 protein was detected by Western blot，and HIF mRNA was tested by RT-PCR. ［Results］ Low dose of Rhizoma Chuanxiong had a killing effect on PG-BE1 stem-like cells，and inhibited the expression of ABCG2 protein(P<0.05). In addition，low dose Rhizoma Chuanxiong also inhibited the expression of both HIF-1α mRNA and protein. ［Conclusion］ Low dose Rhizoma Chuanxiong can inhibit cancer stem cells，which is associated with down-regulation of HIF-1α expression and improvement of tumor hypoxic microenvironment. |
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