| 陈寒露,陈海滔,徐 超.姜黄素对结肠癌细胞株NKD2及CXCR4基因表达影响的研究[J].中国肿瘤,2016,25(4):297-302. |
| 姜黄素对结肠癌细胞株NKD2及CXCR4基因表达影响的研究 |
| The Effects of Curcumin on Expression of NKD2 and CXCR4 Gene in SW620 Colon Cancer Cell |
| 投稿时间：2015-10-24 |
| DOI：10.11735/j.issn.1004-0242.2016.04.A011 |
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| 中文关键词: 姜黄素 SW620结肠癌细胞 NKD2 CXCR4 |
| 英文关键词:curcumin SW620 colon cancer cell NKD2 CXCR4 |
| 基金项目:浙江省医学卫生平台计划（2015DTA005） |
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| 中文摘要: |
| 摘 要：［目的］ 观察姜黄素对SW620细胞及裸鼠NKD2、CXCR4表达水平的影响。［方法］ 通过MTS检测姜黄素对SW620细胞活性的影响；运用Western blot、Real-time qPCR检测不同浓度姜黄素对SW620细胞及转染NKD2 siRNA后SW620细胞内NKD2、CXCR4蛋白和mRNA表达的影响。雄性BALB/c裸鼠8只，随机分成对照组与姜黄素组，每组4只。采用皮下接种SW620细胞。造模后第7d开始给对照组与姜黄素组分别注射对照液和100mg/kg·d浓度的姜黄素悬液，连续用药14d。给药后检测瘤体体积，通过Real-time qPCR检测瘤体组织内NKD2及CXCR4表达水平。［结果］ 姜黄素能明显抑制SW620细胞的增殖活性，显著性上调SW620细胞内NKD2表达及下调CXCR4的表达，且呈剂量依赖性。转染后发现能上调姜黄素处理后的肿瘤细胞CXCR4 的表达水平。体内实验表明姜黄素抑瘤率为30.69%。通过qPCR发现瘤体组织中NKD2的表达量增加，CXCR4的表达量显著性降低。［结论］ 姜黄素均能从体内外明显抑制结肠癌细胞SW620的生长，其作用机制可能通过上调抑制基因NKD2的表达，抑制Wnt信号通路，进而下调肿瘤细胞CXCR4表达，从而起到抑制肿瘤侵袭转移的作用。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effects of curcumin on the expression of NKD2 and CXCR4 in SW620 cell and mice respectively. ［Methods］ The effects of curcumin on SW620 cells’ activity were detected by MTS assay. The protein and mRNA expression of NKD2 and CXCR4 in the SW620 cells treated with different concentrations of curcumin by Western blot and Real-time qPCR. Eight male BALB/c mice were randomly divided into control group and curcumin group，4 in each. A mice of SW620 colon cancer was established by subcutaneously inoculating tumor cell suspension. Mice in the control group were intraperitoneally injected control liquid and 100mg/kg·d of curcumin suspension was injected in the curcumin group，both for 14 days. Detecting tumor volume after administration and then detecting the mRNA expression of NKD2 and CXCR4 in tumor tissues by Real-time qPCR.［Results］ Curcumin inhibited the activity of SW620 cells significantly. Curcumin could up-regulate the expression of NKD2 and down-regulate the expression of CXCR4 in SW620 cell with a dose-dependent manner. After transfection，the expression level of CXCR4 in SW620 cells treated with curcumin was increased. In vivo experiments，the inhibition rate of curcumin was 30.69%. The expression of NKD2 increased and the expression of CXCR4 significantly decreased in the tumor tissue by Real-time qPCR. ［Conclusion］ Curcumin can significantly inhibit the growth of SW620 colon cancer cell in vitro and in vivo. Curcumin may inhibit Wnt pathway by up-regulating the expression of NKD2，then inhibit the expression of CXCR4 in tumor cells and inhibit tumor invasion and metastasis eventually. |
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