| 邝少松,郑佳琳,杨 林.CyclinE及相关基因在人原发性肝癌中的表达及其意义[J].中国肿瘤,2016,25(2):143-149. |
| CyclinE及相关基因在人原发性肝癌中的表达及其意义 |
| Expression and Significance of CyclinE and Related Genes in Hepatocellular Carcinoma |
| 投稿时间：2015-08-13 |
| DOI：10.11735/j.issn.1004-0242.2016.02.A013 |
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| 中文关键词: 原发性肝癌 CyclinE CDK4 VEGF c-myc p53 |
| 英文关键词: |
| 基金项目:广东省科技计划资助项目（2012B05050006） |
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| 摘要点击次数: 2018 |
| 全文下载次数: 778 |
| 中文摘要: |
| 摘 要：［目的］ 研究CyclinE、CDK4、VEGF、c-myc及p53基因在肝癌中的表达及其意义。［方法］ 采用荧光实时定量PCR技术、免疫组织化学技术及蛋白免疫印迹技术对肝癌组织、自身癌旁组织的CyclinE、CDK4、VEGF、c-myc及p53基因的表达水平进行检测。［结果］ 肝癌组织中的CyclinE、CDK4、VEGF、c-myc及p53 mRNA的表达水平均高于自身癌旁组织（P 均< 0.05）；CyclinE蛋白在肝癌组织中的阳性表达率为59.52%；CDK4蛋白在肝癌组织中的阳性表达率为40.48%；VEGF蛋白在肝癌组织中的阳性表达率为35.71%；c-myc蛋白在肝癌组织中的阳性表达率为7.62%；p53蛋白在肝癌组织中的阳性表达率为52.38%；而在癌旁组织不表达或呈微弱表达。相比于中、高分化肝癌患者，低分化癌组织中的各蛋白的阳性表达率均显著升高（CyclinE：94.74% vs 30.43%；CDK4：47.37% vs 34.78%；VEGF：42.11% vs 30.43%；c-myc：57.89% vs 39.13%；p53：63.16% vs 43.48%），且差异有统计学意义（均P<0.05）。相比于没有发生肝外转移的肝癌患者，有肝外转移的癌组织中的各蛋白的阳性表达率均显著升高（CyclinE：81.25% vs 46.15%；CDK4：68.75% vs 23.08%；VEGF：43.75% vs 30.77%；c-myc：62.50% vs 38.46%；p53：62.50% vs 46.15%），且差异有统计学意义（P均<0.05）。［结论］ CyclinE、CDK4、VEGF、c-myc及p53基因在人肝细胞癌组织中的表达呈阳性，且阳性表达程度与肿瘤分化程度和有无肝外转移相关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the expression and medical significance of CyclinE，CDK4，VEGF，c-myc and p53 gene in hepatocellular carcinoma (HCC). ［Methods］ Genetic levels of these genes in HCC and adjacent tissues were detected by quantitative real-time PCR(Q-PCR)，and the protein expression levels were detected by Western Blot and immunohistochemistry. ［Results］ Each of those genes expression in HCC tissues was significantly higher than that in adjacent tissues(P<0.05). The positive expression rates of CyclinE，CDK4，VEGF，c-myc and p53 were 59.52%，40.48%，35.71%，7.62% and 52.3%，respectively. The positive expression rate of each gene in poorly differentiated hepatocellular carcinoma was significantly higher than that in moderately and well-differentiated carcinoma(CyclinE：94.74% vs 30.43%；CDK4：47.37% vs 34.78%；VEGF：42.11% vs 30.43%；c-myc：57.89% vs 39.13%；p53：63.16% vs 43.48%，P<0.05). And the positive expression of each gene was also significantly higher in the tissues of hepatocellular carcinoma metastasis than that in tissues without metastasis ( CyclinE：81.25% vs 46.15%；CDK4：68.75% vs 23.08%；VEGF：43.75% vs 30.77%；c-myc：62.50% vs 38.46%；p53：62.50% vs 46.15%，P<0.05).［Conclusion］ CyclinE，CDK4，VEGF，c-myc and p53 genes are highly expressed in tissues of HCC both in genetic and protein level and correlated with tumor differentiation and metastasis. |
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