| 刘江涛,谭立君,李慧军.RNA干扰抑制Skp2表达对人喉癌细胞系Hep-2的影响[J].中国肿瘤,2016,25(1):63-69. |
| RNA干扰抑制Skp2表达对人喉癌细胞系Hep-2的影响 |
| Effect of RNA Interference Inhibiting Skp2 Expression on Human Laryngeal Carcinoma Cell Line Hep-2 |
| 投稿时间：2015-07-10 |
| DOI：10.11735/j.issn.1004-0242.2016.01.A012 |
|
 |
| 中文关键词: 喉鳞状细胞癌 细胞S期激酶相关蛋白2 RNA干扰 慢病毒 |
| 英文关键词:laryngeal squamous cell carcinoma S-phase kinase associated protein 2 RNA interference lentivirus |
| 基金项目:黑龙江省教育厅基金项目（11521193） |
|
| 摘要点击次数: 2142 |
| 全文下载次数: 742 |
| 中文摘要: |
| 摘 要： ［目的］ 研究RNA干扰抑制Skp2表达对喉癌鳞状细胞p27表达、细胞增殖和凋亡的作用。［方法］ 利用脂质体将重组质粒转染Hep-2细胞，用慢病毒系统建立干扰Skp2基团的稳定细胞株；荧光实时定量PCR和Western blot法检测转染细胞株中Skp2和p27mRNA及其蛋白表达； 采用MTT法和流式细胞仪检测转染细胞的增殖和凋亡情况。［结果］ 通过慢病毒siRNA技术，Skp2可持续稳定抑制Hep2喉癌细胞，siRNA可诱导抑制Skp2，增加p27表达，降低细胞增殖，增加喉癌细胞的凋亡。［结论］ 靶向Skp2基因的 siRNA 对Hep-2细胞的抑制作用可能是通过上调 p27 基因水平来实现的，Skp2是基因疗法治疗喉癌的有利靶点。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of RNA interference Skp2 suppression on p27 expression，cellular proliferation，apoptosis in human laryngeal carcinoma cell line Hep-2. ［Methods］ Recombinant plasmids were transfected into Hep-2 cell with liposome. To construct the stable cell line interfered Skp2 gene with lentivirus system. Fluorescent realtime quantitated PCR and Western blot were used to detect expression of mRNA and protein Skp2 and p27 in transfected cell lines. MTT and flow cytometer were used to detect the proliferation and apoptosis in transfected cell lines. ［Results］ Skp2 was stably suppressed in Hep2 laryngeal carcinoma cells by lentivirus siRNA technology. The siRNA-induced suppression of Skp2 increased p27 expression ，decreased cellular proliferation and increased apoptosis in human laryngeal carcinoma cells. ［Conclusion］ The inhibitation effect of siRNA targeting Skp2 on Hep-2 cell are probably derived from the increased expression of p27 gene，Skp2 is a viable target in the gene therapy treatment for human laryngeal carcinoma. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |