| 郭丽丽,郭胜利,郭 炜.食管鳞状细胞癌中ADAMTS1mRNA及其蛋白表达[J].中国肿瘤,2015,24(7):598-602. |
| 食管鳞状细胞癌中ADAMTS1mRNA及其蛋白表达 |
| Expression of ADAMTS1 mRNA and Protein in Esophageal Squamous Cell Carcinoma |
| 投稿时间：2014-12-02 |
| DOI：10.11735/j.issn.1004-0242.2015.07.A013 |
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| 中文关键词: 食管鳞状细胞癌 ADAMTS1 RT-PCR 免疫组织化学 |
| 英文关键词:esophageal squamous cell carcinoma a disintegrin and metalloprotease with thrombospondin motifs 1 RT-PCR immunohistochemistry |
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| 中文摘要: |
| 摘 要：［目的］ 评价含凝血酶敏感素基序的去整合素金属蛋白酶1（a disintegrin and metalloproteinase with thrombospondin motifs 1，ADAMTS1）mRNA及其蛋白表达在食管鳞状细胞癌（esophageal squamous cell carcinoma，ESCC）发生发展中的意义。［方法］ 应用RT-PCR方法及免疫组织化学SP方法分别检测ESCC组织及癌旁正常组织中ADAMTS1 mRNA及其蛋白表达的情况。［结果］ ①ADAMTS1mRNA在ESCC组织中表达量显著性低于癌旁正常组织（0.394±0.123 vs 0.895±0.276，P<0.01）；ADAMTS1mRNA在无淋巴结转移组中的表达量显著性低于淋巴结转移组（0.298±0.102 vs 0.482±0.157，P<0.05）。②ESCC组织中ADAMTS1蛋白的阳性表达率显著性低于癌旁组织（38.9% vs 94.4%，P<0.01）。［结论］ ADAMTS1异常表达可能与ESCC的发生、发展及淋巴结转移密切相关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the significance of expression of a disintegrin and metalloproteinase with thrombospondin motifs 1(ADAMTS1) mRNA and protein in esophagus squamous cell carcinoma (ESCC). ［Methods］ Reverse transcriptase polymerase chain reaction method (RT-PCR) and immunohistochemistry method were used respectively to detect expression of ADAMTS1 mRNA and protein in ESCC tumor tissues and corresponding normal tissues. ［Results］ ①The expression of ADAMTS1 mRNA in ESCC tumor tissue was significantly lower than that in corresponding normal tissue (0.394±0.123 vs. 0.895±0.276，P<0.01). The ADAMTS1mRNA level in the ESCC tumor with lymph node metastasis was significantly higher than that without lymph node metastasis (0.482±0.157 vs. 0.298±0.102，P<0.05). ②The positive expression rate of ADAMTS1 protein in ESCC tumor tissues was significantly lower than that in corresponding normal tissue (38.9% vs. 94.4%，P<0.01). ［Conclusions］ Aberrant expression of ADAMTS1 might be closely related to carcinogensis，occurrence and lymph node metastasis of ESCC. |
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