| 孔守芳,戴淑真,吕 腾.慢病毒介导Stathmin-1基因沉默对宫颈Hela细胞p53蛋白的影响[J].中国肿瘤,2015,24(3):246-249. |
| 慢病毒介导Stathmin-1基因沉默对宫颈Hela细胞p53蛋白的影响 |
| Lentivirus-mediated Stathmin-1 Silencing on p53 Protein in Cervical Hela Cells |
| 投稿时间：2014-09-19 |
| DOI：10.11735/j.issn.1004-0242.2015.03.A016 |
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| 中文关键词: Stathmin-1 p53蛋白 Hela细胞 慢病毒 细胞增殖 |
| 英文关键词:Stathmin-1 p53 protein Hela cell Lentivirus proliferation |
| 基金项目:青岛市科技发展计划（13-1-3-118-jcl） |
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| 中文摘要: |
| 摘 要：［目的］ 研究宫颈癌Hela细胞中Stathmin-1表达对p53蛋白的影响及对细胞增殖的作用。［方法］ 构建Stathmin-1 siRNA慢病毒载体，包装重组慢病毒，感染宫颈癌Hela细胞株，沉默Stathmin-1表达为干扰组，采用导入阴性片段的慢病毒感染Hela细胞作为对照组。MTT法检测细胞增殖，蛋白印迹法（Western-blot）检测细胞p53蛋白的表达。［结果］ 感染重组慢病毒及阴性病毒后，Stathmin-1基因干扰组Hela细胞增殖24h后较对照组抑制明显（P<0.05）。与对照组相比，干扰组中p53蛋白表达明显减少（P<0.05）。［结论］ 宫颈癌Hela细胞中Stathmin-1影响p53蛋白表达，沉默Stathmin-1可抑制细胞增殖。 |
| 英文摘要: |
| Abstract：［Purpose］ To study the influences Stathmin-1 on p53 protein expression in Hela cells and the role on cells proliferation. ［Methods］ Short hairpin RNA(shRNA) targeting Stathmin-1 was designed and lentivirus carried the shRNA infected Hela cells as interference group. Control group cells were infected with lentivirus carried negative fragment. After recombinant lentivirus infected Hela cells，MTT assay was used to detect the status of proliferation. Western blot was used to analyze p53 protein expression. ［Results］ With STMN-1 silencing，MTT assay results suggested Hela cells proliferation was suppressed obviously in interference group compared with control group(P<0.05).The p53 protein expression in interference group was decreased(P<0.05). ［Conclusion］ Stathmin-1 expression can influence p53 protein expression in cervical Hela cells. Silencing Stathmin-1 can restrain cervical cancer cells proliferation. |
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