| 宋仕茂,王 云,骆志国.靶向干扰Spy1表达增强食管癌化疗敏感性的研究[J].中国肿瘤,2015,24(3):241-245. |
| 靶向干扰Spy1表达增强食管癌化疗敏感性的研究 |
| Study on Targeted Depletion of Spy1 Sensitizes Esophageal Cancer Cells to Cisplatin |
| 投稿时间：2014-06-10 |
| DOI：10.11735/j.issn.1004-0242.2015.03.A015 |
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| 中文关键词: 食管癌 Spy1 RNA干扰 顺铂 化疗敏感性 |
| 英文关键词:esophageal carcinoma Spy1 siRNA cisplatin chemosensitivity |
| 基金项目: |
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| 摘要点击次数: 2028 |
| 全文下载次数: 933 |
| 中文摘要: |
| 摘 要：［目的］ 探讨抑制Spy1表达后食管癌细胞对化疗药物顺铂敏感性的变化。［方法］ 在食管癌Eca-109细胞中转染靶向Spy1的siRNA，采用RT-PCR和Western Blot检测Spy1的表达情况；MTT法检测抑制Spy1表达后对Eca-109细胞生长和对顺铂化疗敏感性的影响；流式细胞术检测干扰Spy1对细胞周期的影响。［结果］ 转染Spy1 siRNA后，食管癌Eca-109细胞中Spy1的mRNA和蛋白水平明显下降；MTT结果显示顺铂对Eca-109细胞的半数抑制浓度(50% inhibiting concentration，IC50)由4.56±1.23μg/ml降至1.12±0.09μg/ml；转染Spy1 siRNA联合顺铂IC50处理使细胞存活率由(64.7±3.8)%降至(46.8±4.2)%；细胞周期更多阻滞于G0/G1期(P＜0.05)。［结论］ 靶向干扰Spy1表达可抑制食管癌细胞的生长，增强顺铂对肿瘤细胞的杀伤作用，其机制与细胞阻滞于G0/G1期有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the siRNA-mediated inhibition of Spy1 gene on the chemosensitivity to cisplatin of Eca-109 cells. ［Methods］ Eca-109 cells were transfected with Spy1 siRNA. RT-PCR and Western Blot analysis were used to verify the inhibitory effect of siRNA against Spy1．MTT assay was conducted to determine the effects of Spy1 depletion on the chemosensitivity of Eca-109 cells. ［Results］ Spy1 was down-regulated in Eca-109 cells transfected with specific siRNA. The down-regulation of Spy1 decreased the IC50 values of Eca-109 cells to cisplatin from 4.56±1.23μg/ml to 1.12±0.09μg/ml. Spy1 depletion combined with ciaplatin administration decreased the cell survival rate from(64.7±3.8)% to (46.8±4.2)%. Flow cytometry showed that cell cycle of the Spy1 siRNA group was obviously blocked in G0/G1 phase than other groups.［Conclusion］ Targeted depletion of Spy1 gene can inhibit proliferation of human esophageal cancer Eca-109 cells，and improve the sensitivity of cells to cisplatin. Induction of G0/G1 cell cycle arrest might be a related mechanism. |
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