| 林 妙,姚庆华,汪玉琪.氧化应激在姜黄素诱导肺癌细胞凋亡中的作用[J].中国肿瘤,2015,24(3):229-233. |
| 氧化应激在姜黄素诱导肺癌细胞凋亡中的作用 |
| Role of Oxidative Stress on Curcumin Induced Lung Cancer Cell Apoptosis |
| 投稿时间：2014-10-17 |
| DOI：10.11735/j.issn.1004-0242.2015.03.A013 |
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| 中文关键词: 姜黄素 肺癌 凋亡 氧化应激 热休克蛋白70 |
| 英文关键词:curcumin lung cancer apoptosis oxidative stress heat shock protein70 |
| 基金项目: |
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| 摘要点击次数: 2260 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨姜黄素诱导肺癌A549细胞凋亡中氧化应激的作用。［方法］ 将不同浓度(5~40μM)的姜黄素作用于肺癌细胞株A549细胞，以MTT法检测姜黄素不同浓度（5~40μM）在不同时间点（3、6、12、24、48 h）对A549细胞的抑制增殖率，流式细胞术检测细胞凋亡率；DCFH-DA探针染色流式检测细胞内活性氧(reactive oxygen species，ROS)的变化，硫代巴比妥酸法检测丙二醛（malondialdehyde，MDA），Western blot检测Bcl-2、Bax和HSP70的表达。［结果］ 20μM姜黄素作用细胞12h和24h时，细胞增殖抑制率分别为32.17% 和52.16%。姜黄素对A549细胞的生长抑制作用呈剂量和时间依赖性。A549细胞凋亡率和坏死率随着姜黄素的浓度增高而增加。经姜黄素处理后，细胞内活性氧显著性降低，丙二醛活性下降，HSP70活性下降，Bax/Bcl-2比值增高。［结论］姜黄素可调节肺癌细胞内氧化还原状态，从而促进肿瘤细胞凋亡。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of curcumin on the proliferation and apoptosis in A549 lung cancer cells. ［Methods］ Different concentrations(5~40μM) of curcumin treated with A549 cells in different times（3、6、12、24、48h）. The cell viability was determined by MTT assay. The apoptosis was detected by flow cytometry. The level of reactive oxygen species(ROS) was determined with DCFH-DA by flow cytometry，and malondialdehyde(MDA) was detected by malondialdehyde barbituric acid method. Bcl-2，Bax，HSP70 activity was assayed by Western blot. ［Results］ 20μM curcumin treated with cell for 12h and 24h，the cell proliferation inhibition rate was 32.17% and 52.16%，respectively. The inhibitory effect of curcumin on A549 cells was in a dose-and time-dependent manner. Apoptosis and necrosis rate of A549 cells increased with concentration of curcumin. After curcumin treated，intracellular ROS significantly reduced，MDA activity declined，then HSP70 activity declined，Bax/Bcl-2 ratio increased. ［Conclusion］ Curcumin could regulate intracellular REDOX state and promote the tumor cell apoptosis in lung cancer. |
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