郑翠玲,姜 萍,杜 莹.不同EDTA盐抗凝血对恶性肿瘤患者网织红细胞参数的影响[J].中国肿瘤,2014,23(11):961-964. |
不同EDTA盐抗凝血对恶性肿瘤患者网织红细胞参数的影响 |
Effects of Different EDTA Salts as Anticoagulants on Reticulocyte Parameters of Blood in Patients with Malignancy |
投稿时间:2014-07-30 |
DOI:10.11735/j.issn.1004-0242.2014.11.A019 |
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中文关键词: EDTA盐 肿瘤 网织红细胞参数 |
英文关键词:EDTA salts tumor reticulocyte parameters |
基金项目:国家自然科学基金资助项目(81201357) |
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中文摘要: |
摘 要:[目的] 探讨不同EDTA盐抗凝血对恶性肿瘤患者网织红细胞(RET)参数的影响。[方法] 实验采用配对研究,分别使用含EDTA-K2和EDTA-K3抗凝剂的真空采血管采集80例肿瘤患者全血标本进行RET参数,包括网织红细胞(RET#),网织红细胞千分比(RET‰),低荧光强度网织红细胞比率(LFR),中荧光强度网织红细胞比率(MFR),高荧光强度网织红细胞比率(HFR)和网织红细胞未成熟分数(IRF)的测定。采用配对t检验分析配对EDTA抗凝血中RET参数值的差异。[结果] EDTA-K2抗凝血的RET参数(RET#=0.0805±0.0305,RET‰=19.2±7.7,LFR=91.8±6.3,MFR=7.8±5.2,HFR=2.2±4.1,IRF=10.0±8.6)高于EDTA-K3抗凝血的RET参数(RET#=0.0628±0.0275,RET‰=15.4±6.9,LFR=89.9±8.4,MFR=6.7±3.9,HFR=1.7±2.9,IRF=7.8±5.5),且差异具有统计学意义(P均<0.05)。EDTA-K2和 EDTA-K3抗凝血的RET参数在不同癌种患者之间比较差异无统计学意义(P>0.05)。[结论] 不同EDTA盐抗凝血对恶性肿瘤患者RET参数的检测结果存在一定影响。 |
英文摘要: |
Abstract:[Purpose] To investigate the effects of anticoagulanted blood of different EDTA salts on reticulocyte(RET) parameters in patients with malignant tumor. [Methods] Comparative experiments were carried out,80 whole blood samples were collected with different anticoagulants(EDTA-K2 and EDTA-K3,respectively) from patients with malignant tumors,and RET parameters including reticulocytes(RET#),the permillage of reticulocyte(RET‰),low fluorescent reticulocyte ratio(LFR),medium fluorescent reticulocyte ratio(MFR),high fluorescent reticulocyte ratio(HFR), and the immature reticulocyte(IRF)) were measured. Paired t-test was used to analyze the differences of RET parameters value between paired EDTA-anticoagulated blood.[Results] RET parameters of EDTA-K2 anticoagulated blood (RET#=0.0805±0.0305,RET‰=19.2±7.7,LFR=91.8±6.3,MFR=7.8±5.2,HFR=2.2±4.1,IRF=10.0±8.6) were higher than those of EDTA-K3 anticoagulated blood (RET#=0.0628±0.0275,RET‰=15.4±6.9,LFR=89.9±8.4,MFR=6.7±3.9,HFR=1.7±2.9,IRF=7.8±5.5) (P all<0.05). There were no statistical differences between EDTA-K2 anticoagulated blood from patients with different malignant tumors(P>0.05). Similarly,there were no statistical differences between EDTA-K3 anticoagulated blood samples obtained from patients with different malignant tumors(P>0.05). [Conclusions] Anticoagulanted blood of different EDTA salts would have some influence on the detection results of RET parameters in patients with malignant tumors. |
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