| 刘 唯,吴素慧,史晓峰.RNA干扰沉默MIF基因对宫颈癌SiHa细胞上皮细胞间质变的影响[J].中国肿瘤,2014,23(11):956-960. |
| RNA干扰沉默MIF基因对宫颈癌SiHa细胞上皮细胞间质变的影响 |
| Effects of Silencing Migration Inhibitory Factor Gene by RNA Interference on Expression of EMT-associated Protein in Human Cervical Carcinoma SiHa Cell |
| 投稿时间：2014-02-24 |
| DOI：10.11735/j.issn.1004-0242.2014.11.A018 |
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| 中文关键词: RNA干扰 上皮细胞间质转化 巨噬细胞移动抑制因子 宫颈癌 |
| 英文关键词:RNA interference epithelial-mesenchymal transition(EMT) MIF cervical cancer |
| 基金项目:山西省自然科学基金资助项目（2010011047-3）； |
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| 中文摘要: |
| 摘 要：［目的］ 探讨巨噬细胞移动抑制因子(MIF)对人宫颈癌SiHa细胞上皮间质转化（EMT）的影响。［方法］ 构建并鉴定重组真核表达质粒Genesil-MIF siRNA，转染人宫颈癌细胞SiHa细胞。RT-PCR检测各组细胞中MIF、E-cadherin、Vimentin mRNA表达水平。免疫细胞化学法组检测E-cadherin、Vimentin蛋白表达水平。［结果］ 重组质粒Genesil-MIF siRNA成功构建并转染至人宫颈癌SiHa细胞。RT-PCR法证实实验组细胞中E-cadherin mRNA相对高表达（P＜0.05），而Vimentin、MIF mRNA相对低表达（P＜0.05）。免疫细胞化学法证实实验组细胞中E-cadherin蛋白表达水平升高，Vimentin蛋白表达水平明显降低。［结论］ MIF沉默通过促进SiHa细胞中E-cadherin表达，抑制Vimentin表达从而抑制EMT发生。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the effect of RNA interference(RNAi) targeting macrophage migration inhibitory factor (MIF) on epithelial-mesenchymal transition(EMT)-associated proteins in human cervical carcinoma SiHa cells.［Methods］ Recombinant eukaryotic expression plasmid pGenesil-MIF siRNA was constructed and identified，then transfected into human cervical cancer cells SiHa using a liposome approach. Real-time PCR was used to detect the silencing efficacy of MIF mRNA and the expression of E-cadherin、Vimentin mRNA．The protein expression of E-cadherin and Vimentin was detected by immunocytochemistry.［Results］ The recombinant plasmid pEGFP-N1-MIF was constructed successfully and transfected into SiHa cells. In experimental group，the mRNA expression of Vimentin decreased，whereas E-cadherin expression increased. Immunocytochemical staining showed that expression of E-cadherin increased，whereas Vimentin expression decreased.［Conclusion］ RNA interference of MIF suppresses epithelial-mesenchymal transition in SiHa cells by decreasing the expression of E-cadherin and increasing the expression of Vimentin. |
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