| 邢晓静,谷小虎,马天飞.RIN1过表达对人胃腺癌MKN28细胞增殖、侵袭、迁移和凋亡的影响[J].中国肿瘤,2014,23(7):601-607. |
| RIN1过表达对人胃腺癌MKN28细胞增殖、侵袭、迁移和凋亡的影响 |
| Effect of RIN1 Overexpression on Proliferation，Invasion，Migration and Apoptosis of Human Gastric Adenocarcinoma Cell Line MKN28 |
| 投稿时间：2013-11-29 |
| DOI：10.11735/j.issn.1004-0242.2014.07.A014 |
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| 中文关键词: RIN1 人胃腺癌MKN28细胞 增殖 侵袭 迁移 凋亡 |
| 英文关键词:RIN1 human gastric adenocarcinoma cell line MKN28 proliferation invasion migration apoptosis |
| 基金项目:辽宁省科技公关项目（2012225016）；辽宁省自然科学基金（201102111）；国家自然科学基金项目（81201968） |
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| 中文摘要: |
| 摘 要：［目的］ 研究RIN1过表达对人胃腺癌MKN28细胞增殖、侵袭、迁移和凋亡的影响。［方法］ ①采用Western blot 法和免疫荧光法检测RIN1在MKN28细胞中表达；②采用Burd-U标记检测MKN28细胞高表达RIN1后细胞增殖能力变化；③采用Transwell法、细胞划痕法检测MKN28细胞高表达RIN1后细胞侵袭和迁移能力变化；④采用AV/PI染色法、Hoechst染色法检测MKN28细胞高表达RIN1后细胞凋亡情况。［结果］ ①RIN1在MKN28细胞中呈高表达，表达量是空载对照组的3.22倍，且主要分布在细胞质中；②Burd-U染色结果显示，RIN1高表达MKN28细胞的阳性率显著性升高，与空载对照组相比为75.6%±5.4% vs 25.6%±1.1%(P<0.01)；③RIN1高表达MKN28细胞的侵袭和迁移能力明显增强，与空载对照组相比分别为122.0±7.0 vs 65.00±2.52(P<0.01)和54.22%±3.45% vs 38.6%±2.45%(P<0.01)；④RIN1高表达MKN28细胞株细胞凋亡数显著性减少，与空载对照组相比为21.63%±2.60% vs 8.07%±1.60%(P<0.01)。［结论］RIN1过表达促进MKN28细胞增殖、侵袭和迁移，抑制MKN28细胞凋亡；RIN1可能是介导胃癌发生的癌基因之一。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of RIN1 overexpression on proliferation，invasion，migration and apoptosis of human gastric adenocarcinoma cell line MKN28. ［Methods］ RIN1 expression in pcDNA3.1(+)/RIN1/ MKN28 cells were identified by Western blot and Immunofluorescence. The effect of RIN1 overexpression on proliferation，invasion，migration and apoptosis of MKN28 cells were analyzed by Brd-U assay，Transwell chamber and Scratch Wound Healing，AV/PI dye method and Hoechst staining，respectively.［Results］ RIN1 was highly expressed in pcDNA3.1、(+)/RIN1/MKN28 cells. The quantity of RIN1 expression on pcDNA3.1(+)/RIN1/MKN28 cells was 3.22 times of pcDNA3.1(+)/MKN28 cells，and RIN1 mainly distributed in the cytoplasm. Compared with pcDNA3.1(+)/MKN28 cells，the proliferation ability of pcDNA3.1(+)/RIN1/MKN28 cells (75.6%±5.4% vs 25.6%±1.1%，P<0.01) was significantly enhanced，and the migrated cell number (122.0±7.0 vs 65.00±2.52，P<0.01) and the migrated rate of pcDNA3.1(+)/RIN1/MKN28 cells(54.22%±3.45% vs 38.6%±2.45%，P<0.01) were markedly increased and the apoptotic rate of pcDNA3.1(+)/RIN1/MKN28 cells(8.07%±1.60% vs 21.63%±2.60%，P<0.01) was dramatically reduced.［Conclusion］ RIN overexpressed in MKN28 cells is capable of promoting proliferation，invasion，migration and inhibiting apoptosis，and RIN1 may be one of oncogenes to take part in the occurrence of gastric cancer. |
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