| 花永虹,胡巧英,朴永锋.细梗香草皂甙对鼻咽癌CNE-2细胞的体外抗瘤作用[J].中国肿瘤,2014,23(7):597-600. |
| 细梗香草皂甙对鼻咽癌CNE-2细胞的体外抗瘤作用 |
| The Antitumor Effect of Capilliposide on Nasopharyngeal Cancer Cell Line CNE-2 in vitro |
| 投稿时间：2014-01-22 |
| DOI：10.11735/j.issn.1004-0242.2014.07.A013 |
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| 中文关键词: 鼻咽肿瘤 细梗香草皂甙 细胞增殖 凋亡 |
| 英文关键词:nasopharyngeal neoplasm capilliposide cell proliferation apoptosis |
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| 摘要点击次数: 2112 |
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| 中文摘要: |
| 摘 要：［目的］ 观察细梗香草皂甙抗人鼻咽癌CNE-2细胞的效应。［方法］ 细梗香草皂甙与CNE-2细胞共培养，采用MTT法检测细胞增殖，软琼脂实验检测细胞集落形成情况，流式细胞仪检测细胞凋亡。 ［结果］ 不同浓度细梗香草皂甙作用于CNE-2细胞后，细胞存活率显著性降低，48h细梗香草皂甙IC50为7.4μg/ml。CNE-2细胞经8μg/ml细梗香草皂甙处理后，软琼脂集落形成能力下降，集落形成数为119±11个，比对照组（297±24个）低；细梗香草皂甙组的凋亡率达16.43%±3.1％，而对照组为9.34%±2.3%。 ［结论］ 细梗香草皂甙对鼻咽癌CNE-2细胞株有抑制增殖和诱导凋亡的作用。 |
| 英文摘要: |
| Abstract：［Purpose］ To observe the antitumor activity of capilliposide on human nasopharyngeal cancer cell lines CNE-2.［Methods］ CNE-2 cells were cultured with different concentrations with capilliposide. Cell proliferation were measured by MTT assay，colony formation by a soft agar assay and apoptosis by flow cytometry. ［Results］ The survival rate of CNE-2 cells was significantly decreased after incubation with capilliposide，and the IC50 in 48 hours was 7.4μg/ml. After 8μg/ml concentrations treatment，the number of colony-formation in soft agar of the CNE-2 cells was 297±24，which was lower than that in control group( 119±11). Apoptosis rate was 16.43%±3.1％ in capilliposide group and 9.34%±2.3% in control group.［Conclusion］ Capilliposide plays a role in growth inhibition and inducing apoptosis in human nasopharyngeal cancer cell line CNE-2. |
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