| 蔡锦威,汤海标,邵喜英.川楝素对乳腺癌细胞的生长抑制作用[J].中国肿瘤,2014,23(4):342-345. |
| 川楝素对乳腺癌细胞的生长抑制作用 |
| Effects of Toosendanin on Growth Inhibition in Human Breast Cancer Cells |
| 投稿时间：2013-12-25 |
| DOI：10.11735/j.issn.1004-0242.2014.04.A015 |
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| 中文关键词: 川楝素 乳腺癌 MDA-MB-453 细胞增殖 细胞凋亡 |
| 英文关键词:toosendanin breast cancer MDA-MB-453 cell proliferation apoptosis |
| 基金项目:浙江省自然科学基金（Y2101312）；浙江省医药卫生科技计划（2010QNA006） |
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| 摘要点击次数: 1998 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨川楝素对人乳腺癌MDA-MB-453细胞作用及其机制。［方法］ 取对数生长期的MDA-MB-453细胞，0、6.25、12.50、25.00、50.00、100.00nmol/L川楝素处理，绘制生长曲线。川楝素 0、12.5、50nmol/L处理72h后，MTS法检测川楝素对乳腺癌细胞增殖的影响，流式细胞仪检测川楝素对乳腺癌细胞凋亡和细胞周期的影响。［结果］ 川楝素对乳腺癌细胞有增殖抑制作用，并呈时间剂量依赖性（P＜0.01）。MDA-MB-453细胞48h、72h、96h的IC50分别为17.25、22.20和135.69nmol/L。流式细胞分析表明，川楝素诱导乳腺癌细胞的凋亡呈浓度依赖性（P＜0.01），且阻滞细胞在S期（P＜0.01），以0、12.50、50.00nmol/L川楝素处理乳腺癌细胞72h后，MDA-MB-453细胞早期凋亡率分别为3.21%、9.64%和20.19%，MDA-MB-453细胞处于细胞周期S期细胞占20.98%、35.92%和45.02%。［结论］ 川楝素对乳腺癌MDA-MB-453细胞增殖具有抑制作用，其作用机制可能与诱导细胞凋亡和引起S期阻滞有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of toosendanin on proliferation in human breast cancer MDA-MB-453 cells and its possible mechanism.［Methods］ Logarithmic growth phase of MDA-MB-453 cells were selected. The growth curve was made with 0，6.25，12.50，25.00，50.00，100.00nmol/L toosendanin treated. After toosendanin 0，12.5，50nmol/L for 72h，cell proliferation in breast cancer cells was measured by MTS assay，cell apoptosis and cell cycle was detected by flow cytometry，the expression of Caspase3 and PARP were detected with Western blot assay.［Results］ Toosendanin showed an inhibitory effect with a time-dose-dependent manner in breast cancer MDA-MB-453 cells（P＜0.01）.The IC50 of MDA-MB-453 at 48h、72h、96h was 17.25、22.20、135.69nmol/L. Flow cytometric analysis showed that toosendanin induced apoptosis of breast cancer cell in a dose-dependent manner (P<0.01) and arrested the cell in S phase(P<0.01). After 0，12.50，50.00 nmol/L toosendanin treated for 72h，early apoptosis rate was 3.21%，9.64%，and 20.19% in MDA-MB-453 cell respectively. S phase of the cell cycle accounted for 20.98%，35.92% and 40.05% in MDA-MB-453 cell.［Conclusion］ Toosendanin may play an important effect of inhibiting the growth of MDA-MB-453 cell，the mechanism might be related to inducing apoptosis and arresting S-phase cell cycle. |
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