| 闫 超,刘 昊,彭海声.慢病毒介导CD-TK融合基因转染神经干细胞后杀伤C6胶质瘤的研究[J].中国肿瘤,2014,23(1):63-67. |
| 慢病毒介导CD-TK融合基因转染神经干细胞后杀伤C6胶质瘤的研究 |
| Effect of Neural Stem Cells Modified by CD-TK Gene Mediated by Lentivirus on Glioma Cells |
| 投稿时间：2013-03-22 |
| DOI：10.11735/j.issn.1004-0242.2014.01.A015 |
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| 中文关键词: CD-TK融合基因 神经干细胞 胶质瘤 基因治疗 |
| 英文关键词:CD-TK gene neural stem cells glioma gene therapy |
| 基金项目: |
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| 摘要点击次数: 1998 |
| 全文下载次数: 1035 |
| 中文摘要: |
| 摘 要：［目的］ 观察慢病毒介导的CD-TK融合基因转染神经干细胞（NSCs）对C6胶质瘤生长的抑制作用以及疗效。［方法］ 利用慢病毒介导的大肠杆菌胞嘧啶脱氨酶(CD)和单纯疱疹病毒胸苷激酶(HSV-TK)融合基因转染NSCs，植入C6胶质瘤模型。用流式细胞仪技术检测C6胶质瘤生长凋亡情况。［结果］ 成功构建 CD-TK 基因转染NSCs模型。实验组坏死细胞（17.80%）、早期凋亡细胞及晚期凋亡细胞（33.30%）较对照组增加。CDglyTK-NSC+GCV/5-Fc治疗组肿瘤体积为9.36±2.35mm3，小于其他处理组及对照组的肿瘤体积（P<0.05）。［结论］转染NSCs的CD-TK 基因对C6胶质瘤细胞有抑制作用，抗肿瘤效果明显，优于单一的自杀基因系统。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the inhibition effect and efficacy of neural stem cells modified by CD-TK gene mediated by lentivirus in the treatment of glioma. ［Methods］ CD-TK gene was introduced into NSCs by lentivirus-mediated transfection and implanted into the model of C6 glioma. FCM was used to observe the apoptosis of glioma in vivo.［Results］ NSCs/CD-TK model was successfully constructed. Necrotic cells，apoptotic cells in treatment group were significantly higher than those in other groups（P<0.05）.Tumor volume in CDglyTK-NSC+GCV/5-Fc group (9.36±2.35mm3)was smaller than that in other groups（P<0.05）. ［Conclusion］ NSCs/CD-TK signifi-cantly inhibited the growth of C6 cells with a better anti-tumor efficacy than CD-TK suicide gene. |
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