| 唐 秋,郭 勇,胡巧英.紫龙金对鼻咽癌裸鼠移植瘤放射增敏作用的研究[J].中国肿瘤,2013,22(9):737-742. |
| 紫龙金对鼻咽癌裸鼠移植瘤放射增敏作用的研究 |
| Radiosensitizing Effect of Zilongjin on Human Nasopharyngeal Carcinoma CNE-1 Transplantation Tumor in Nude Mice |
| 投稿时间：2013-05-06 |
| DOI：10.11735/j.issn.1004-0242.2013.09.A014 |
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| 中文关键词: 紫龙金 鼻咽癌 放射增敏 p16 cyclinD1 |
| 英文关键词:Zilongjin nasopharygeal carcinoma radiosensitizing p16 cyclinD1 |
| 基金项目:浙江省中医药科技计划资助项目 (2010B591127) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨紫龙金对鼻咽癌细胞系裸小鼠移植瘤放射增敏作用及机制。［方法］ 建立CNE-Ⅰ裸小鼠移植瘤模型，观察空白对照组、紫龙金组、单纯照射组和紫龙金加照射组肿瘤体积变化，并计算抑瘤率和放射增敏比（SER）；免疫组化检测肿瘤组织中p16和cyclinD1蛋白的表达；逆转录聚合酶链反应（RT-PCR）检测 p16和cyclinD1 mRNA的表达。［结果］ 紫龙金加照射组抑瘤率明显高于其他组，SER(E/O)为2.45，提示紫龙金有放疗增敏效果。免疫组化分析法和RT-PCR显示，紫龙金组和紫龙金加照射组与空白对照组及单纯照射组相比，p16蛋白和p16 mRNA表达显著上调（P<0.05）；cyclinD1蛋白和cyclind1 mRNA表达显著下调（P<0.05）。［结论］ 紫龙金对鼻咽癌细胞系CNE-Ⅰ裸小鼠移植瘤有放射增敏作用，其增敏作用机制可能与p16表达上调、cylinD1表达下调有关。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate radiosensitization of Chinese herbal medicine Zilongjin on the transplantation tumor of nasopharyngeal carcinoma CNE-1 and its mechanism. ［Methods］ The transplantation tumor models of nasopharyngeal carcinoma CNE-1 were established，and divided into control group，alone radiation group，Zilongjin group and Zilongjin plus radiation group. The changes of tumor size in various groups were observed and the sensitizing enhancement ratio(E/O) was calculated. The expressions of p16 protein and cyclinD1 protein in tumor tissue were examined by immunohistochemitry staining. RT-PCR was applied to detect p16 mRNA and cyclinD1 mRNA.［Results］ The tumor inhibition rate in Zilongjin plus radiation group was higher than that in other groups. E/O was 2.45，that means Zilongjin had the effects on radiosensitization. Immunohistochemitry staining and RT-PCR results showed that compared with control group and alone radiation group，the expression of p16 protein and p16 mRNA was up-regulation and expressions of cyclinD1 protein and cyclinD1 mRNA was down-regulation in Zilongjin group and Zilongjin plus radiation group(P<0.05). ［Conclusion］ Zilongjin has radiosensitization on CNE-Ⅰ transplantation tumor. Its mechanisms of radiosensitization might relate to p16 up-regulation and cyclinD1 down-regulation. |
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