| 马 瑛,彭芝兰,陈 欣.MASPIN、HPA在卵巢癌血管生成中的作用[J].中国肿瘤,2013,22(7):586-591. |
| MASPIN、HPA在卵巢癌血管生成中的作用 |
| The Role of MASPIN、HPA Expression on Angiogenesis in Ovarian Cancer |
| 投稿时间：2013-03-01 |
| DOI：10.11735/j.issn.1004-0242.2013.07.A015 |
|
 |
| 中文关键词: 卵巢癌 丝氨酸蛋白酶抑制剂 乙酰肝素酶 血管生成 |
| 英文关键词:ovarian cancer mammary serpin heparanase angiogenesis |
| 基金项目:四川省卫生厅科研立项（070206） |
|
| 摘要点击次数: 2296 |
| 全文下载次数: 1026 |
| 中文摘要: |
| 摘 要: ［目的］ 研究丝氨酸蛋白酶抑制剂(MASPIN)、乙酰肝素酶(HPA）在卵巢癌的表达和血管生成的作用。［方法］ 采用免疫组化法对31例正常卵巢组织、35例上皮性卵巢癌标本进行MASPIN、HPA表达检测，CD34抗体标记血管内皮细胞计数微血管密度（MVD）。MASPIN真核表达质粒体外转染卵巢癌SKOV3细胞，用细胞免疫化学及半定量逆转录聚合酶链反应（RT-PCR）方法检测转染后卵巢癌细胞中HPA蛋白和 HPA mRNA表达变化。［结果］ MASPIN在正常卵巢生发上皮细胞和卵巢癌细胞的阳性表达率分别为16.1%(5/31)和57.1%(20/35)(P<0.05)。HPA蛋白在正常卵巢生发上皮细胞未见表达，在卵巢癌细胞的阳性表达率为85.7%(30/35)(P<0.05)；MVD在卵巢癌中增高(P<0.05)。MASPIN蛋白表达与卵巢癌病理类型、临床分期和腹水相关(P<0.05)。HPA蛋白表达与卵巢癌淋巴结转移和腹水相关（P<0.05）。细胞免疫组化及RT-PCR方法均证实转染MASPIN cDNA的SKOV3细胞中HPA表达在各组间差异无统计学意义(P>0.05）。 ［结论］ MASPIN在卵巢癌的发展过程中可能发挥着肿瘤抑制作用，并具有抑制血管生成作用。HPA表达与卵巢癌的恶性生物学行为密切相关。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the expression of mammary serpin(MASPIN)、heparanase(HPA) in ovarian cancer and the role in angiogenesis. ［Methods］ The expression of MASPIN and HPA was detected immunohistochemically in specimens of normal ovary(n=31) and epithelial ovarian cancer(n=35)．Microvessel density(MVD) was counted by endothelial cells immunostained with anti-CD34 monoclonal antibody. The recombinant plasmid pcDNA3-MASPIN was stable transfected into ovarian cancer cell line SKOV3. The expressions of HPA protein and HPA mRNA were determined by immunocytochemistry and RT-PCR. ［Results］ The positive expression rate of MASPIN was 57.1% in ovarian cancer and 16.1% in normal tissues（P<0.05). The positive expression rate of HPA was 85.7%(30/35) in ovarian cancer and negative expression in normal tissuess（P<0.05）. MVD increased in ovarian cancer as compare with control group(P<0.05)． MASPIN expression was correlated to pathological type，stage and astites(P<0.05). HPA expression was correlated to lymph node involvement and ascites(P<0.05). The expression of HPA in SKOV3-MASPIN cell after transfected by recombinant plasmid pcDNA3-MASPIN was not significantly different among all the groups both by immunocytochemistry and RT-PCR.［Conclusions］ MASPIN might play a role in inhibiting tumor and angiogenesis in ovarian cancer. HPA expression correlates to malignant biological behaviors in ovarian cancer. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |