| 李 茵,温 颖,郑东翔.人工合成抗菌肽对口腔肿瘤细胞增殖、侵袭抑制及促凋亡作用[J].中国肿瘤,2013,22(6):466-472. |
| 人工合成抗菌肽对口腔肿瘤细胞增殖、侵袭抑制及促凋亡作用 |
| Effect of Artificial Antimicrobial Peptides on Proliferation，Metastasis and Apoptosis in Oral Tumor Cell Lines |
| 投稿时间：2013-01-21 |
| DOI：10.11735/j.issn.1004-0242.2013.06.A201304144 |
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| 中文关键词: 人工合成抗菌肽 口腔肿瘤细胞系 细胞凋亡 细胞增殖 迁移 侵袭 |
| 英文关键词:artificial antimicrobial peptides oral tumor cell lines proliferation apoptosis invasion metastasis |
| 基金项目:北京市自然科学基金资助项目(7123214) |
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| 摘要点击次数: 2001 |
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| 中文摘要: |
| 摘 要: ［目的］ 体外观察不同浓度的人工合成抗菌肽(artificial antimicrobial peptides，AMPs)对口腔肿瘤细胞系的增殖、侵袭抑制及促凋亡作用。［方法］ 合成AMPs并配置溶液，将AMPs溶液（50μg/ml~200ug/ml）加至肿瘤细胞培养体系中，收获培养后细胞以流式细胞分析法（Annexin-V/PI染色）进行细胞凋亡检测，并通过BrdU掺入实验以及MTS法进行细胞增殖检测。通过细胞迁移以及侵袭实验评价AMPs对口腔肿瘤细胞系迁移及侵袭能力的影响。［结果］ AMPs可诱导口腔肿瘤细胞系SACC-83及Tca8113凋亡。在AMPs 200μmol/L浓度，中晚期凋亡比率分别为(33.89±16.74)%、(32.47±13.53)%，高于PBS对照组的中晚期凋亡比率(4.34±1.08)%和(5.76±1.43)%（P均＜0.05），同时AMPs对肿瘤细胞促凋亡作用的效果与其浓度呈剂量依赖特点。BrdU掺入实验及MTS法发现AMPs可显著性抑制口腔肿瘤细胞系SACC-83及Tca8113的细胞增殖，当AMPs浓度为50μmol/L时对人口腔肿瘤细胞系SACC-83和Tca8113细胞增殖活性有明显的抑制作用，AMPs对人口腔肿瘤细胞系SACC-83和Tca8113细胞增殖抑制作用的IC50分别为60.38μM和55.35μM。低浓度（50μmol/L）AMPs还可显著性抑制SACC-83和Tca8113细胞的迁移以及侵袭。［结论］ AMPs体外对口腔肿瘤细胞系SACC-83及Tca8113有明显的促进凋亡作用，并抑制肿瘤细胞的增殖、迁移及侵袭。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the effect of artificial antimicrobial peptides (AMPs) on proliferation，invasion，metastasis and apoptosis in oral tumor cell line in vitro. ［Methods］ Synthesis AMPs and dissolving the AMPs into PBS. The solution of AMPs (50μg/ml~200ug/ml) was added to the tumor cell culture system. The tumor cells apoptosis was detected by flow cytometry analysis method (Annexin-V/PI staining) ，and proliferation was examined by BrdU and MTS. Transwell migration assay and invasion assay were used to assess the ability of oral tumor cells migration and invasion treated by AMPs. ［Results］ The AMPs could induce the oral tumor cells apoptosis. In the concentration of AMPs 200μmol/L，the percent of late stage apoptosis in oral tumor cells SACC-83 and Tca8113 were (33.89±16.74)% and (32.47±13.53)% respectively，which was significantly higher than those in PBS control group［(4.34±1.08)% and（5.76±1.43）%］（P＜0.05). While the effect of AMPs on inducing apoptosis of tumor cells showed dose-effect with the AMPs concentration. Evaluating by BrdU incorporation assay and the MTS proliferation assay，AMPs also inhibited the proliferation in oral tumor cell lines(SACC-83 and Tca8113). When the AMPs concentration of 50μmol/L，the human oral tumor cell lines SACC-83 Tca8113 cell proliferation was inhibited significantly. The cell proliferation IC50 of AMPs on human oral cancer cell lines SACC-83 and Tca8113 were 60.38μM and 55.35μM respectively. The low concentration of AMPs（50μmol/L） also significantly inhibited the migration and invasion in SACC-83 and Tca8113 cell.［Conclusions］ The AMPs can significantly induce the apoptosis ， and effectively inhibit the proliferation，invasion and metastasis of oral tumor cell lines SACC-83 and Tca8113 in vitro. |
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