| 谢长生,徐丽君.红参含药血清对人乳腺癌MCF?鄄7细胞增殖和细胞周期的影响[J].中国肿瘤,2013,22(4):291-294. |
| 红参含药血清对人乳腺癌MCF?鄄7细胞增殖和细胞周期的影响 |
| Effect of Serum Containing Radix Ginseng Rubra on Cell Prolife-ration and Cell Cycle in Human Breast Cancer Cell Line MCF-7 |
| 投稿时间：2012-10-05 |
| DOI：10.11735/j.issn.1004-0242.2013.04.A201301020 |
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| 中文关键词: 红参 MCF-7细胞；细胞凋亡；细胞周期 |
| 英文关键词:Radix Ginseng Rubra MCF-7 cells apoptosis cell cycle |
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| 中文摘要: |
| 摘 要：［目的］ 研究红参含药血清对人乳腺癌MCF-7细胞增殖和细胞周期的影响。［方法］ 采用MTT法观察红参含药血清对人乳腺癌细胞MCF-7的增殖活性。流式细胞术检测人乳腺癌细胞MCF-7的细胞周期、细胞凋亡率。［结果］ 红参低剂量含药血清对MCF-7细胞有促增殖作用，24、48、72h时增殖率分别为105.9%、115.9%和121.5%。而高剂量对MCF-7细胞表现为抑制作用。红参低剂量组使MCF-7细胞S期DNA含量比例明显增多，高剂量组使细胞周期阻滞在G0/G1期，并能够诱导细胞凋亡，凋亡率达26.86%。［结论］ 红参含药血清在高剂量时抑制MCF-7细胞生长，低剂量时对细胞具有促增殖作用。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the effect of serum containing Radix Ginseng Rubra on cell proliferation and cell cycle in human breast cancer cell line MCF-7. ［Methods］ The cell proliferation of serum containing different concentrations of Chinese medicine“Radix Ginseng Rubra” on human breast cancer cell line MCF-7 in vitro were detected by MTT method， and cell cycle of human breast cancer cell line MCF-7 were examined by flow cytometry.［Results］ Serum containing Radix Ginseng Rubra with lower concentration could make a proliferative effect in MCF-7 cell with proliferation rate 105.9%，115.9%，121.5%，at 24，48，72h respectively，but serum containing Radix Ginseng Rubra with higher concentration inhibited the growth of MCF-7 cells；serum containing Radix Ginseng Rubra with lower concentration could increase DNA percentage in S phase of cell cycle. Serum containing Radix Ginseng Rubra with higher concentration could increase the number of cells in G0/G1 phase and induce cell apoptosis with apoptosis rate of 26.86%. ［Conclusion］ Serum containing Radix Ginseng Rubra with lower concentration could make a proliferative effect in MCF-7 cell，and which with higher concentration could inhibit the growth and induce cell apoptosis in MCF-7 cell. |
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