| 王 建,何志巍.靶向hTERT的小干扰RNA诱导前列腺癌PC3细胞凋亡的机制[J].中国肿瘤,2012,21(3):211-214. |
| 靶向hTERT的小干扰RNA诱导前列腺癌PC3细胞凋亡的机制 |
| Mechanism of Induction of Apoptosis by siRNA Targeting hTERT in Prostatic Cancer PC3 Cells |
| 投稿时间：2011-08-26 |
| DOI： |
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| 中文关键词: 端粒酶逆转录酶 RNA干扰 凋亡 前列腺癌 |
| 英文关键词:hTERT RNAi apoptosis prostatic cancer |
| 基金项目: |
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| 摘要点击次数: 1401 |
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| 中文摘要: |
| 摘 要：［目的］ 研究靶向人端粒酶逆转录酶（hTERT）的siRNA诱导前列腺癌PC3细胞凋亡的分子机制。［方法］ 化学合成的靶向hTERT的siRNA以脂质体法转染PC3细胞；应用寡核苷酸芯片技术分析细胞凋亡相关基因的转录谱变化；应用Western blot检测细胞中hTERT、肿瘤坏死因子相关凋亡诱导配体TRAIL、凋亡抑制基因Bcl-2和胞浆Cyt c的蛋白水平变化；流式细胞术分析细胞凋亡率变化；比色法测定Caspase-3和Caspase-8的相对活性改变。［结果］ 靶向hTERT的siRNA能有效抑制hTERT基因表达；转染hTERT-siRNA 48h后可引起TRAIL的表达上调，Bcl-2表达下调，Cyt c释放增加，Caspase-3活性增强，细胞凋亡率升高。［结论］ 靶向hTERT的小干扰RNA通过活化线粒体信号传导途径诱导PC3细胞凋亡。 |
| 英文摘要: |
| Abstract: ［Purpose］To study the molecular mechanism of induction of apoptosis by siRNA targeting hTERT in prostatic cancer PC3 Cells. ［Methods］ PC3 cells were transfected with chemical synthesized siRNA-hTERT by liposome method. The microarray assay was adopted to explore the transcriptional profiling of apoptosis associated genes. The protein levels of hTERT, TRAIL, Bcl-2 and cytosolic Cyt c were detected by Western blot assay. The apoptosis rate was determined by flow cytometry. The relative activity of Caspase-3 and Caspase-8 was measured by colorimetric assay. ［Results］ The siRNA-hTERT suppressed the expression of hTERT gene significantly. Forty-eight hours after transfection, the expression level of TRAIL was increased, the expression of Bcl-2 was down regulation, the releasing of cytosolic Cytc enhanced, the activation of Caspase-3 increased, and the apoptosis rate increased.［Conclusion］ siRNA-hTERT induces apoptosis of PC3 cells via activating mitochondrial signal transduction pathway. |
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