| 鲁海珍,高 巍,梁 晶.8号染色体倍性改变在原发性胃腺癌诊断中的意义[J].中国肿瘤,2013,22(3):223-228. |
| 8号染色体倍性改变在原发性胃腺癌诊断中的意义 |
| Clinical Significance of Aneuploid of Chromosome 8 in the Dia-gnosis for Gastric Adenocarcinoma |
| 投稿时间：2012-11-26 |
| DOI：10.11735/j.issn.1004-0242.2013.03.A2012339 |
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| 中文关键词: 胃腺癌 荧光原位杂交 染色体 |
| 英文关键词:gastric adenocarcinoma fluorescence in situ hybridization chromosome |
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| 中文摘要: |
| 摘 要：［目的］ 通过研究原发性胃腺癌8号染色体倍性改变，对比胃癌与癌旁组织的差异，探讨其与胃癌病理诊断及临床病理指标的相关性。［方法］ 选取文献报道的胃癌中非整倍体出现频率较高的8号染色体的着丝粒探针，应用荧光原位杂交技术对127例胃腺癌、32例癌旁组织及5例远端胃黏膜组织进行了检测。复阅相应癌和癌旁组织病理切片和临床病理资料，分析所测样本的非整倍体发生率及其临床相关性。［结果］ 胃癌组织的8号染色体非整倍体发生率为68.5%，显著高于癌旁组织（15.6%），具有统计学差异(P＝0.000)。8号染色体倍体改变与患者性别、年龄、部位、大体分型、肿瘤大小、浸润深度、脉管瘤栓、淋巴结转移及病理TNM分期均无显著相关性（P>0.05）。5例癌旁组织检测出8号染色体非整倍体（三体），病理组织学改变为轻度慢性炎症，其中2例伴有轻度肠上皮化生。远端胃黏膜组织8号染色体均表现为正常倍体数（二体）。［结论］ 8号染色体倍体改变为胃癌的诊断提供了候选分子细胞遗传学指标，可能是胃癌发生的早期遗传学改变之一，对早期诊断具有一定提示意义。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the clinical significance of aneuploid of chromosome 8 in gastric adenocarcinoma(GAC) and cancer adjacent tissues，and to investigate the correlation between clinical features and pathology.［Methods］ The aneuploid of chromosome 8 was detected by fluorescence in situ hybridization(FISH) in 127 cases with GAC tissues，32 cases with adjacent tissues and 5 cases with distal normal tissues. We selected centromere probe cen8 specific for chromosome 8，which frequently amplified in GAC according to the references. The clinicopathological features in 127 GAC patients were reviewed. Data were analyzed by SPSS11.5 statistical software for Windows. ［Results］ The results of FISH showed that 68.5% of cen8 were aneuploid in GAC tissues which was significantly higher than that in adjacent tissues (15.6%) (P=0.000). No correlation existed between aneuploid of cen8 and gender，age，localization，macroscopic type，tumor size，grade，Lauren classification，lymph node metastasis and clinical stage(P>0.05).The triploid of chromosome 8 was detected in 5 cases with cancer adjacent tissues, which had mild chronic inflammation in pathological histology, and 2 cases of them with mild intestine epithelium. Five cases with distal normal mucosa were all diploid.［Conclusions］ Detection of aneuploid of cen8 may be helpful GAC diagnosis in molecular genetics level and early diagnosis. |
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